Lung cancer is the leading cause of
cancer death among men and women in
North America.
1
In advanced non–small-cell
lung cancer, chemotherapy offers symptomatic re-
lief and modest improvement in survival
2
; respons-
es are brief, with a median time to progression of
three to five
months. Second-line chemotherapy
with docetaxel can prolong survival after platinum-
based therapy for non–small-cell lung cancer.
3,4
However, there is at present no defined role for
third-line
chemotherapy. The futility of offering
third-line chemotherapy was demonstrated by Mas-
sarelli et al.,
5
who reported a response rate of only
2 percent and a median survival of four months.
Sheph
erd et al.
6
showed that among patients treat-
ed with docetaxel after the
failure of two
or more
chemotherapy regimens, survival
was identical to
that among patients treated with supportive care.
The epidermal growth factor receptor (EGFR)
family is part of a complex signal-transduction net-
work that is central to several critical cellular pro-
cesses. Since EGFR is often found in non–small-
cell lung cancer cells,
7,8
it has been the focus of
efforts
to develop new agents that target the EGFR
pathway. Erlotinib (Tarceva, OSI Pharmaceuticals)
and
gefitinib (Iressa, AstraZeneca) inhibit the ty-
rosine kinase activity of EGFR and have been stud-
ied
extensively.
9-12
In randomized phase 2 trials of
gefitinib (Iressa Dose Evaluation in Advanced Lung
Cancer [IDEAL] 1 and 2),
10,11
the tumors of 10 to
20 percent of patients who were previously treat-
ed with platinum-based regimens responded, and
in a phase 2 trial of erlotinib among previously
treated patie
nts with non–small-cell lung cancer in
which 10
percent or more of the cells expressed
EGFR, the response rate was 12.3 percent.
12
These
promising rates are perhaps higher than those pos-
sible with other forms of chemotherapy,
3-6
but it
is unknown whether treatment with an EGFR inhib-
itor
prolongs survival. For this reason, the National
Cancer Institute of Canada Clinical Trials Group
(NCIC CTG) conducted a trial (BR.21) to compare
erlotinib with placebo after the failure of standard
chemotherapy for non–small-cell lung cancer. The
inclusion of a control group receiving placebo was
considered ethical in view of the lack of benefit from
further chemotherapy after the failure of standard
treatment.
5,6
study design
This international, phase 3, randomized, double-
blind, placebo-controlled trial of erlotinib after the
failure of first-line or second-line chemotherapy
for non–small-cell lung cancer was designed by the
NCIC CTG. Patients were randomly assigned in a
2:1 ratio to receive oral erlotinib at a dose of 150 mg
daily or placebo. Randomization was performed
centrally by Applied Logic Associates (Houston),
with the use of the minimization method.
13
Pa-
tients were stratified according to center, Eastern
Cooperative Oncology Group performance status
(0 or 1 vs. 2 or 3, with higher scores indicating
greater impairment), best response to prior thera-
py (complete or partial response vs. stable disease
vs. progressive disease), number of prior regimens
received (one vs. two), and exposure to prior plati-
num therapy (yes vs. no).
The primary end point was overall survival.
Secondary end points included progression-free
survival, overall response rate (complete and par-
tial), duration of response, toxic effects, and qual-
ity of life. Responses were assessed with the use of
the Response Evaluation Criteria in Solid T
umors
(RECIST),
14
and toxic effects were assessed accord-
ing to
the Common Toxicity Criteria of the
Nation-
al Cancer Institute (version 2.0). The European Or-
ganization for Research and Treatment of Cancer
(EORTC) quality-of-life questionnaire (QLQ-C30)
and the quality-of-life questionnaire for patients
with lung cancer (QLQ-LC13) were used to evalu-
ate p
atients’ quality of life.
The protocol was approved by the ethics review
boards
at all participating institutions, and all pa-
tients provided written informed consent. Support
was provided by the NCIC and OSI Pharmaceuti-
cals. Data
were collected, managed, and analyzed
by the
NCIC CTG, and the manuscript was written
by membe
rs of the NCIC CTG. OSI Pharmaceuti-
cals reviewed the final manuscript and provided
comments on it. Confidentiality was maintained by
both the NCIC CTG and OSI Pharmaceuticals. The
study chair, Dr. Shepherd, and the physician coordi-
nator, Dr.
Seymour, reviewed all the data and con-
firmed their co
mpleteness and accuracy.
l
methods
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n engl j med
353;2
www.nejm.org
july
14, 2005
erlotinib in previously treated non–small-cell lung cancer
125
eligibility criteria
Patients 18 years of age or older with an Eastern
Cooperative Oncology Group (ECOG) performance
status between 0 and 3 were eligible in the presence
of documented pathological evidence of non–small-
cell lung cancer. The patients had to have received
one or two regimens of combination chemother-
apy and not be eligible for further chemotherapy.
Patients 70
years of age or older may have received
therapy with one or two single agents. Patients had
to have recovered from any toxic effects of therapy
and were randomly assigned to the study treatment
at least 21 days after chemotherapy (14 days after
treatment with vinca alkaloids or gemcitabine) and
7 days after radiation. Adequate hematologic and
bioc
hemical values were required.
Patients with prior breast cancer, melanoma, or
hypernephroma were ineligible, as were those with
other malignant diseases (except basal-cell skin
cancers) within the preceding five years. Other ex-
clusion
criteria were symptomatic
brain metasta-
ses, clinically significant cardiac
disease
within one
year, ventricular arrhythmias
requiring med
ication,
and clinically significant ophthalmologic or gastro-
intestinal
abnormalities.
study procedures
Within seven days before randomization, a history
and physical examination were obtained and hema-
tologic and biochemical testing, chest radiography,
and assessments of toxic effects and quality of life
were obtained. Computed tomographic scans of
the chest and abdomen were obtained within 28
days before randomization. For a patient to be eval-
uated for a response, at least one measurable lesion
was required, but measurable disease was not man-
datory for eligibility. Only patients with measur-
able disea
se were included in the analyses of com-
plete or partial response.
Administration of the study medication was to
start within two days after randomization. For
grade 2 diarrhea, loperamide was recommended
without reduction of the dose of erlotinib. For
grade 3 diarrhea, the study treatment was with-
held until the
diarrhea was grade 1 or less, and then
erlotinib at a dose of 100 mg daily was started. For
grade 1
or 2 rash, treatment modification was not
recommended. For grade 3 rash, treatment was
withheld, the rash was treated symptomatically, and
erlotinib at a dose of 100 mg daily was restarted
when t
he rash was grade 1 or less.
History taking, physical examination, and he-
matologic and biochemical testing were performed
every four weeks, and radiologic investigations ev-
ery eight weeks. Patients’ quality of life was evaluat-
ed every four weeks in countries with validated ver-
sions of the questionnaires.
egfr expression
Separate written consent for optional tissue bank-
ing and correlative studies was obtained. EGFR ex-
pression was determined with the use of immuno-
histochemistry in a central laboratory that used
Dako
kits (DakoCytomation). Positivity was defined
as more than 10 percent of cells staining at any in-
tensity for EGFR.
statistical analysis
The trial was designed to detect, with 90 percent
power
and a two-sided type I error of 5 percent, a
33 percent improvement in median survival from
four months as estimated in the placebo group. For
the final analysis, 582 deaths were required and
were
projected to occur with a sample size of 700
patients enrolled over a period of 14 months with
6 months of follow-up. The required number of
deaths
had occurred by January 2004, and the data-
base was locked as of April 23, 2004. There was no
interim analysis. Tumor responses were validated
centrally for the first 333 patients in the trial.
The stratified log-rank test, accounting for strat-
ification factors
at randomization (except center)
and EGFR protein expression (positive vs. negative
vs. unknown), was used to compare progression-
free
survival and overall survival between treatment
groups. Exploratory forward stepwise regression
analys
es with the use of the Cox model were per-
formed to adjust for treatment effect and to iden-
tify prog
nostic factors for progression-free survival
and overall survival. Candidate covariates includ-
ed EGFR expression, stratification factors (except
center), sex, age (60 years or less vs. more than 60
years), race or ethnic group (Asian vs. others), prior
radiotherapy (yes vs. no), histologic subtype of can-
cer (adenocarcinoma vs. others), and smoking sta-
tus (smoker vs. nonsmoker vs. unknown). Race was
self-reported or
determined by study personnel and
was not based on country of domicile. Fisher’s exact
test was used to
compare response rates between
levels of potential p
redictors and rates of toxic ef-
fects between t
reatments. Times to deterioration
(a 10-point increase from the baseline score) for
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